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In either case the conclusion is that in the frozen state the polypeptide structure of the putative binding sites has changed, becoming unable to host ANS or to enhance its fluorescence yield, as when the probe surrounding changes from rigid to mobile (26)
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Relative to azurin (WT), which is a small tightly packed polypeptide, the intensity enhancement is generally larger (Fig
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The C-terminal hydrophobic peptide was deleted by insertion of a stop codon to obtain a soluble protein, and the loop 103-136 was replaced by three histidines to facilitate purification
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3 and 4 are invariant with the changes in the disk concentration in the range explored, thus the unfolding rate is not significantly affected by the lipoprotein concentration
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