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Parameter A, which reflects the spectral shape of the intrinsic Trp fluorescence (31), was obtained by dividing the fluorescence intensity at 320 nm by the intensity at 365 nm
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EI proteins are composed of two domains (6,7): a proteolytically inert N-terminal (EIN), and a proteolytically unstable C-terminal domain (EIC)
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Based on these findings, it is tempting to suggest that the changes monitored by far-UV CD were due to the proximity of residues in the tertiary structure of the whole intact EIC^sup sc^ structure, but not of the isolated domains (and then, in those modeled in this work)
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At room temperature, membranes composed of 1-, 2-dioleoyl (DO) chains, which melt at -18°C, will undergo the gel to liquid crystal phase transition close to 2
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Circular dichroism (CD) and light-scattering melting curves show hysteresis and scan rate dependence, indicating thermodynamically irreversible transition with high activation energy E^sub a^
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Several works implicated importance of particular amino acid residues for insulin aggregation; for example, the bovine insulin, diverging from the human protein at three amino acid positions (A8 Ala^sup Bov^/Thr^sup Hum^, A10 Val^sup Bov^/ Ile^sup Hum^, and B30 Ala^sup Bov^/Thr^sup Hu^), aggregates more easily in vitro (Nielsen et al
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The juxtaposed differences between the populations of fibers grown at 60 and 70°C clearly concern not only the rate of the aggregation process, but different quaternary folds as well
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Abundance and distributionLarvae Larvae of Argentine sandperch occurred between 36[degrees]42'S and 46[degrees]30'S, mainly in coastal waters, in the vicinity of the 50-m isobath (Fig
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