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. (1997) have shown that the rate of reactivation of GR that had been previously unfolded by GdnHCl is not altered by addition of FAD
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. From the distribution of (τ^sup o^^sub m^) values, the shape of the diffusion tensor can be determined (30), whereas the degree of anisotropy can be gauged from the maximum and minimum (τ^sup o^^sub m^)^sub app^ values
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. The complex properties of protein aggregation and amyloid formation require development of sophisticated although simple techniques which can provide detection as well as direct output of structural changes of protein assemblies, e
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. Such an effect would be quite improbable and surprising, should the fiber be produced by a spontaneous association of several free pre-existing protofilaments of the same length
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. This is usually interpreted to mean that the residue in question enjoys the majority of its native contacts at the transition state (Fersht, 2000)
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. In addition, kinetic information on subpopulations interconverting on the microsecond to millisecond timescales can be obtained from a detailed analysis of the FRET histogram peak shapes
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