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Deterministic pressure dissociation and unfolding of triose phosphate isomerase: persistent heterogeneity of a protein dimer
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The folding of ACYI has been characterized to be a three-state process involving an inactive dimeric intermediate with molten globule-like characteristics (18,19)
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Positive crosspeaks could be found at (1649 cm^sup -1^, 1636 cm^sup -1^), and negative crosspeaks were located at (1649 cm^sup -1^, 1617 cm^sup -1^) and (1658 cm^sup -1^, 1617 cm^sup -1^)
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In Flory's approach, a range of chain properties including R^sub g^, the average end-to-end distance (R^sub e^), the hydrodynamic radius (R^sub h^), the second virial coefficient (B^sub 2^), and the osmotic pressure (II) are calculated as series expansions in terms of the parameter z = N (T - Θ/T) (39,40,45)
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two peaks with different thermodynamical properties are observed, one corresponding to EIN (with pH-independent T^sub m^, values) and a second corresponding to EIC (with pH-dependent T^sub m^-values) (6)
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The excitation light, provided by a Cermax xenon lamp (LX150UV, ILC Technology, Sunnyvale, CA), is selected (λ^sub ex^ = 350 nm for ANS and 285 nm for azurin fluorescence, respectively, 6 nm bandwidth) by a 0
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The C-terminal hydrophobic peptide was deleted by insertion of a stop codon to obtain a soluble protein, and the loop 103-136 was replaced by three histidines to facilitate purification
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In this case, however, stabilization of the native state is most probably due to a decrease in free energy of the native state; phosphate, like sulfate, is a salting-out ion that stabilizes folded protein structures and favors aggregation and precipitation
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Deriving the activation barriers assuming a two-state exchange is likely to be a significant simplification, as the conformational landscape may be much more complex
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Disk fusion is accompanied by dissociation of lipid-poor monomolecular apoA-2, as indicated by the low-molecular-weight band corresponding to the particle size of ~7
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Six months ago, a cholesterol-lowering drug called Mevacor was set to be reclassified as an over-the-counter medicine, a move that would have made the drug physically and financially available to people like Alston
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It is unclear from our aggregate data if opiates were being used as a substitute for NSAIDs, or if they were increasing for other reasons
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The only victims in these transactions were the people who ended up doing time for making the mistake of thinking Tom Coleman was their friend
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But the suggestion is by no means outlandish
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Planned as a sequel to an impaired driving education program, the workbook contains 69 worksheets to be completed in one of four tracks coordinated with individual counseling
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Like retail pharmacy operators nationwide, Costco's pharmacy team has done what it can to prepare its pharmacists for Medicare Part D
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50 *Second (n=56) SLSI-PCS12 -0
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Making the right high-percentage play is the key to poker," says Williams
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The review also points out that "There is uncertainty and concern regarding the Government's commitment to its Problem Gambling Strategy
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Pun Man On, a retired native of Macao, thinks the city is toodependent on casinos
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Can allow players to have a relatively good idea of which cards remain in the shoe
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Call it zero-sum geopolitics: Their loss is our gain
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To them, the country's vast reserves of oil and natural gas make it a natural ally
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Whereas probability theory calculates odds on the assumption that all relevant facts are specified in the evidence, evidential functions calculate odds on the extent to which all relevant facts are specified in the evidence
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