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. 3 mol of reactive SH group per mol of monomer under native conditions, indicating that one of the thiol groups of GR is partially accessible to DTNB in the folded protein
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. The heat flow versus temperature profile of ACYI contained an asymmetric positive peak arising from an exothermic transition (Fig
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. An additional step of affinity purification was done using a lactosyl-Biogel column to ensure the presence of only the active, refolded molecules in the rSBA sample finally obtained (21)
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. Thermodynamic properties of the fluid and solid phases for inverse power potentials
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. I, The binding of 1-anilino-8-naphthalenesulfonate
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. Spectra from the relaxation-compensated CPMG experiments were integrated and analyzed in the same manner as the R^sub 1^ and R^sub 2^ experiments
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. 4 as the correlation between monocations' concentrations necessary for a 50% transition from coil DNA to compact DNA calculated from corresponding compaction curves on Fig
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. Purified proteins were dissolved in 8 M Urea and refolded by dialysis as described in Gursky and Atkinson (1996)
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. Some independent observations support this hypothesis: 1) back-calculations of hatching date based on daily growth increments from 19 post-transition juveniles collected in northeast Camarones Bay, between 43[degrees]50'S and 44[degrees]43'S, indicated birth dates between February and March (Venerus and Brown, 2003); 2) the collection of one P
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