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due to simultaneous unfolding between the C- and N-terminal domains
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Functional interactions between proteins of the phosphoenolpyruvate:sugar phosphotransferase systems of Bacillus subtilis and Escherichia coli
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Elastase-mediated phosphatidylserine receptor cleavage impairs apoptotic cell clearance in cystic fibrosis and bronchiectasis
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Although for BSA a high binding capacity may be expected from its intrinsic tendency to bind nonpolar and aromatic moieties also in the liquid state, the above ANS binding data do suggest that beyond specific trends of individual polypeptides, multi-subunit proteins are more susceptible than monomers to destabilization and partial unfolding in the frozen state
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1 C), consistent with the absence of such changes in repetitive CD scans
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The high-temperature transition in apoA-2: DMPC disks (solid circles, Fig
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, 2004), and insulin (Bouchard et al
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4, A and B), and the all-atom values are still in the buildup phase throughout the 8-ns trajectories (Fig
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NaHCO^sub 3^ was added as a pH buffer while CO2 was passed continuously through the solution
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Those antibodies were named MT-1, MT-2, and MT-3
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The authors would like to thank Mike Kayser and Jean Pringle for their invaluable advice, help and guidance
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Received 13 January 2003; Accepted after revision 3 February 2004
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My manager [said] only sell two bottles
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