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. In essence, the structure consisted of an α-helix subdomain, which is a four-helix bundle, comprising α-helices 1-4; an α/β subdomain, comprising a β-sandwich, formed by a four-stranded parallel β-sheet (β-strands 1-4) and a three-stranded antiparallel β-sheet (β-strands 1, 5, and 6), and three short helices (α-helices 5-7) that contain the phosphorylation site (His^sup 186^) (Fig
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. Although there were changes in the tertiary structure, EI^sup sc^ retained some, if not all, of its secondary structure, as shown by the increase (in absolute value) of the [Θ] at 222 nm (Fig
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. The tetrameric band at 55 kD originates from the amount of unfolded monomers that have not yet been misfolded/misassembled and can refold instead of forming aggregates during the pH jump (from pH 2 to pH 7
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