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This leads us to further understanding of intermediate protein structures in the unfolding process
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When the sample was heated up to 90°C, immediately cooled, and reheated, the reheating scan had no thermal effect over the temperature range of 30°C-90°C, which suggested that the thermal denaturation of ACYI was fully irreversible
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Proteins in high concentrations of denaturants, such as 8 M urea or 6 M GdnCl, behave like chains in good solvents (3)
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The raw data were corrected for pre- and posttransition slopes
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Aliquots of the aggregation reaction was withdrawn and assayed to avoid adverse effects of probe binding during the reaction
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