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The recomhinant form also shows emission maxima at 327 nm for the native stale and 357 nm in the completely denatured state (Fig
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The whole emission spectrum with a wavelength increment of I nm was acquired approximately within 30 s
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With respect to corresponding CD spectra we observed for both types of samples (i
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In solution, this helix is disrupted in the middle (2), which allows N- and C-terminal domains of both apo-CaM and Ca^sup 2+^/CaM to tumble almost independently of each other (3-5)
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A challenge faced by all these models is that the most successful search for inherent features of tertiary structure that correlate with folding rates has found that the topological measure of "contact order" is far more closely related than, for example, molecular weight itself in the case of "two-state" folders (Plaxco et al
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