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Further observations on the electrical properties of hemoglobin-bound water
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Fluorescence measurements were done on a Jobin Yvon Horiha fluorometer (Jobin Yvon Spex, Cedex, France) in a 1-cm cell connected to a circulation water hath (Julabo, Seelbach
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8 be tested experimentally? Fluctuations for a chain of length N will lead to cavities that are large enough to allow for the free diffusion of a smaller chain of length nContacts are hierarchical and average topologies are independent of sequence Two residues are said to he in contact if there are at least two atoms (including hydrogen atoms), one from each residue, within a 6-[Angstrom]; distance of each other
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The other major change in a fluorescence signal upon conformational changes of the protein is related to solvent relaxation, which determines the magnitude of the stokes shift
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In addition, all thermal transitions in NDSB solutions exhibit also the missing reversibility as observed for pure buffer solutions
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(9) recorded MSDs for the huperzine A/AChE complex that were also larger than the MSDs for the free enzyme in a number of picosecond simulation periods during a total simulation time of 1 ns
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Before initiation of the aggregation reaction, TTR is in the form of unfolded monomers
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RESULTSMeasurement of protein-membrane associations under pressurePrevious studies of model membranes underpressure suggest that the primary effect of pressure is in the compressible hydrocarbon region, and the application of pressure increases the gel to liquid crystal phase transition by a constant that depends primarily on the lipid chains (Li and Kay, 1977)
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For nonionic interactions, the dominant pressure effect resides in void volumes between the protein and the membrane surface
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On the contrary, it seems that a single protofilament may act as a lateral template or scaffold for small insulin particles, which would constitute a neighboring subcordlike features in the fibril shown in Fig
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It is this protein that finds its way to cheese
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Our data on T^sub g^ of the IPP polymers (9 to 13°C) are consistent with reference 3, which reported that the T^sub g^ of IPP polymer ranges from about -30 to +20°C and depends on tacticity and thermal history
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Even in the strong El Nino year of 1997-98, nearshore water temperature during the winter larval development period was only slightly outside this range
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We note that variations of [gamma] as a function of solvent could potentially produce shifts in peak position in the histograms
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