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RESULTS AND DISCUSSION The thermal dependence of ACYI activity was determined by measuring the catalytic activity of the samples equilibrated at given temperatures tor 30 min
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Given access to contact density maps for the denatured state (EV limit) and contact maps for native states, one can make a qualitative judgment regarding the folding process by computing difference contact density maps between the native and denatured states
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These observations provide the necessary impetus for developing an accurate statistical thermodynamics framework for understanding how polypeptides respond to increasing concentrations of denaturants to yield ensembles that converge upon the EV limit description
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1°C/min) gives rise to less pronounced aggregation during the thermal unfolding transition as compared to higher heating rates; and (2), the lowest protein concentration (7 µg/ml) reveals a less-pronounced aggregation also for a higher heating rate (1°C/min), whereas higher protein concentrations studied with the higher heating rate exhibit more pronounced aggregation
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The drug we selected is an antitrypanosomal agent: diminazene aceturate (also referred to as berenil), the diminazene component of which is known to strongly bind to nucleic acid duplexes by forming hydrogen bonds (24)
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In the vegetal cytoplasm, the aceturate peaks are still smaller than in the animal cytoplasm, concordant with the vegetal cytoplasm being even more stuffed with maternal macromolecules (31,39,40)
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Thus, the relationship between thermal atomic fluctuations and functionally important motions is not straightforward
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This is called "wraparound" binding because the two domains in CaM wrap around the α-helical target (8,9)
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Throughout all experiments, only ultrapure water (resistivity > 18 MΩ cm) was used
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Given the accuracy of the height determination and the x-ray diffraction and small-angle x-ray scattering data (e
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By combining the trajectories of the free and complex forms, further insight into the conformational sampling was extracted
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The carbonate + pyrite globules thus synthesized were heated to, then cooled from, 470 °C in a differential scanning calorimeter at the rate of 20 °C/min in a stream of CO2 at a pressure of 13
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