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Thermodynamic study of the apomyoglobin structure
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Since ACYI can maintain its dimeric structure at a high GdnHCl concentration of 2 M (18), it seems that the dimerization domain was quite stable against denaturation
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The number and locations of nonnative local contacts that are to be broken determine the sets of spatial contacts that are formed upon folding
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For example, milk-fat-globule-factor (MFG)-E8 and growth-arrest-specific-factor 6 (Gas6) are soluble bridging molecules that directly link PS on the apoptotic cell surface with their corresponding phagocyte receptors; [alpha]v[[beta]
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Jamming phase diagram for attractive particles
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However, not only do we find that pressure destabilizes the membrane binding of these G-protein subunits but that identical pressure behavior is seen for Gα^sub s^ even when its palmitoyl chains are removed by reducing agents
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, before the onset of the second calorimetric transition, is fully reversible and leads to no detectable changes in the subsequent DSC scans (data not shown)
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During mix storage even a slight degree of serum separation can produce variable quality because of changes in the level and nature of the total solids as a non-uniform mix goes to the freezer
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However, the herein-observed morphological variation after increasing temperature bears a more profound meaning in light of the ongoing debate as to the identity of the protein's aggregation-prone conformation (completely unfolded, or partly unfolded protein) and our own study, showing that under similar conditions, bovine insulin is substantially more unfolded at 70 than at 60°C (Dzwolak et al, 2003)
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However, when a polymer-solvent system undergoes LLPS prior to crystallization in a certain range of polymer concentration (see Figure 1), the crystallization curve is modified
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