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The second term provides an estimate of the conformational entropy, which opposes chain swelling
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However, at the relatively high CaM concentration (1 mM) used in the NMR experiments, compared to the nanomolar dissociation constant of the complex, all CaM is in the bound state
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The fluorescent dye, 4,6'-diamidino-2-phenylindole (DAPI) was obtained from Wako Pure Chemical Industries (Osaka, Japan)
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In practice, of course, the dimensionality of the folding space is enormous
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The conformational entropy change of gp120/CD4 complexation roughly agrees with the experimental value despite the fact that the contribution from bulk solvent was neglected in our analysis
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The 4WJ ribozyme docks with the highest efficiency, i
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Taken together, the smooth E shifts with changing Mg^sup 2+^ concentration combined with the observed broadening/ tailing at high Mg^sup 2+^ concentration are consistent with the presence of a dynamic equilibrium between the quasi-docked state and an extended undocked state, with a relatively small energy barrier for interconversion (Fig
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