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5 M, an increase in intensity of the fluorescence emission, along with a red shift from 327 to 336 nm was observed (Fig
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Then, one is tempted to ask, is there any relationship among the different functions and structures of the PTS proteins on one side, and the thennodynamic parameters and folding properties on the other? To answer this question, it is necessary to describe the conformational preferences of proteins, where studies of different members of the family have been described
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Although this value of specific volume is required to approximate the viscous behavior at intermediate protein concentrations, the specific volume used for approximating the concentration-dependent osmotic pressure (1
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Although the fluorescence lifetime of ANS is not in the range to enable accurate determination of the rotational correlation time of a large oligomer, ANS anisotropy decay can be used as a measure of aggregation both through point measurements and in a kinetic assay showing limiting anisotropy baselines
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BD/BLx100HD/BLx100Preflexion 3
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RESULTSConfigurational entropy of gp120 and CD4The configurational entropy of various sets of atoms of the free gp120 and CD4 molecules and their complex are shown in Figs
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A threshold of 40 counts (sum of signals from the two channels) was then used to separate fluorescence signals from background, and FRET efficiencies were calculated for each accepted event using the equation E = (1 + [gamma]I^sub d^/I^sub a^)^sup -1^
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