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The enzymatic activity was determined by measuring the absorbance at 238 nm accompanied with hydrolysis of the substrate and using the molar absorption coefficient ε^sub 238^ = 185 M^sup -1^cm^sup -1^ as reported by Kordel and Schneider (5,6), except that chloroacetyl-L-leucine was used instead of pure-L-enantiomorph ultram 50 mg .
Sequence-specific conformational preferences are restricted to local length scales that span five to nine residues ultram 50 mg .
This feature is shared with Gaussian chains (39), although clear differences exist in the magnitude of and correlation between conformational fluctuations ultram 50 mg .
Infrared evidence of a β-hairpin peptide structure in solution ultram 50 mg .
The impact of aggregation on fluorescence emission spectra To investigate how fluorescence emission spectra are affected by aggregation we measured the aggregation directly by the use of elastic light scattering and we analyzed unfolding transitions under different conditions which lead to a different extent of protein aggregation ultram 50 mg .
For this purpose, either a cooling back to room temperature or a removal of GndHCl (by dialysis) has been carried out to measure properties of the enzymes after the denaturing impact was eliminated ultram 50 mg .
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In contrast to reports on DNA binding with monocations, the compaction potentials of monocations determined here correlate well with biological assays that describe the compaction of chromosomes ultram 50 mg .
This makes a direct determination of the rotational correlation time impossible ultram 50 mg .
The spectrum of lipid-free apoA-2 at 25°C is invariant in the protein concentration range from 5 to 20 µg/ml and suggests 30% α-helix (Fig ultram 50 mg .
Seasonal and spatial changes in the larval fish fauna within a large temperate Australian estuary ultram 50 mg .
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Red sea bream (Pagrus major) (Table 1) eggs can easily be distinguished from those of other sparids also found in Japan, such as Acanthopagrus latus, by differences in egg size and spawning seasons, and from those of Evynnis japonica by differences in spawning seasons (Ikeda and Mito, 1988; Kinoshita, 1988; Hayashi, 2000) ultram 50 mg .
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