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. To quantify the secondary structure components, the amide I' band was decomposed into its constituents by curve-titling (based on a combination of Gaussian and Lorentzian functions)
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. The far-UV CD spectrum of EI^sup sc^ at neutral pH showed the features of an α-helical protein with minima at 208 and 222 nm (41,42) (Fig
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. 1), the calculated R^sub h^ of both the protein in solution and its aggregate increased in size and was correlated to the phase separation/precipitation of BLG from aqueous solution at these concentrations and ionic strength (34)
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. Detailed lists of residues are given in Table 1
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. The resultant frequent excursions to this quasidocked structure can facilitate subsequent formation of specific contacts between the two loops, thereby stabilizing the resulting tertiary structure by reducing the entropie penalty for docking
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. Infrared spectroscopy The IR samples were prepared by dissolving ACYI in 100 mM deuterated phosphate saline buffer at a concentration of 50 mg/ml
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. For each of the amino acid sequences shown in Table 1, ensemble averages and conformalional distributions were obtained from an ensemble with a sample size of 10^sup 4^ and the ensembles were generated as discussed above
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. In the EV limit, we lind that irrespective of amino acid sequence, internal distances follow the power law predicted by theory for chains in a good solvent
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. self-association of EI^sup sc^ could be mapped by some of its fluorescence residues (one tryptophan and eight tyrosine residues); and
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. Hence, a quasi-docked conformation could be visited many times during the course of hairpin ribozyme folding, thereby increasing the probability of forming the key tertiary contacts required for stable docking of loops A and B
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