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This investigation aims to complement that initial study by addressing the following issues: i), establish whether the perturbation of protein structure reported for azurin is general to other proteins; ii), inquire on potential correlations between the susceptibility to the ice-induced perturbation and specific features of the macromolecule such as thermodynamic stability, internal flexibility, internal cavities/hydration, and covalent links between strands; and finally iii), test for a specific vulnerability of multimeric proteins to freeze damage that may be linked to the disruption of quaternary structure tramadol withdrawal symptom .
The dependence of the ANS fluorescence intensity, in ice at - 13°C, on the starting ANS concentration in solution (10-200 µM) is shown in Fig tramadol withdrawal symptom .
Mayama and co-workers studied single-molecule compaction of DNA by comparing sodium and potassium chlorides in PEG solutions and noted a large difference in the temperature-dependencies of DNA compaction mediated by these two monocations (Mayama et al tramadol withdrawal symptom .
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