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Thermodynamic data on the conformational stability of proteins can be obtained using several chemical or physical perturbants, including chaotropic agents (guanidine hydrochloride, urea), hydrostatic pressure, and temperature
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The fluorescence emission of GR undergoes a marked red-shift between 1 and 3 M GdnHCl (with a transition midpoint at ~2
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First, this myoglobin solution was adjusted to pH 1
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The genes accumulate mutations that impair their function, and so the unnecessary structures governed by the genes degenerate
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In contrast, the recombinant protein unfolds via an intermediate
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Of course, what we refer to as cavities will actually be filled by solvent and cosolule molecules under denaturing conditions
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The structure of the modeled dimer nicely explained why the solvent accessibility of the Tip^sup 461^ was reduced as the concentration of EI^sup sc^ increased (see before)
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3 A depicts the chemical structure of diminazene aceturate, with its MR signaling proton sites labeled (a-e)
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Measurement of intracellular triglyceride stores by ^sup 1^H spectroscopy: validation in vivo
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The reaction was followed for >300 h
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It should be noted that DCVJ reaches the plateau level faster than ThT and ANS, indicating a strong preference for oligomeric aggregates
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We found no changes in DNS fluorescence over 1-2000 bars, suggesting stable membrane binding even through the phase transition (Fig
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Perhaps the most remarkable example is the determination by fast kinetic experiments that β -lactoglobulin employs a transient helical motif that is entirely nonnative (Park et al
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During cleavage or ligation, the two loops are required to interact, which comes about through a magnesium-dependent folding transition that places the loops in close physical proximity (Murchie et al
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The expression of specific adhesion molecules or surface markers may be altered by the culture conditions used during monolayer expansion
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The Museum of Modern Art opened on Oct
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The intense curriculum may produce stress on the pharmacy student's life
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