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4 strongly supported the conclusion that pretransitional conformation changes occurred at temperatures where the enzyme retained high activity (see Fig
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MATERIALS AND METHODS Materials Tris (hydroxymethyl) aminomethane buffer, [N-[2-hydroxyethyl]piperazine-N'-[2-ethanesulphonic acid]] (HEPES), and guanidinium hydrochloride were from Sigma Chemical (St
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Louis, Missouri Submitted March 31, 2006, and accepted for publication May 31, 2006
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The lowest protein concentration of 7 µg/ml reveals a large red shift with λ^sub max^ of ~345 nm, whereas protein concentrations of 50 µg/ml and 1 mg/ml result in λ^sub max^-values ~340 nm for the unfolded states
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4 ms was used for (180 µm)^sup 3^ voxel spectra, and TE = 6
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In the later stages, the voxels no more provide single cell resolution, but the variation of lipids or other metabolites within a particular region of the developing embryo could still be studied noninvasively and in real time
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Equilibrium cluster formation in concentrated protein solutions and colloids
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All of these monocations collapsed DNA into globules and the concentrations of monovalent salts needed to induce DNA compaction varied greatly
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5 kbar (see Sassaroli et al
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Although the exact time of disk reconstitution varies for apA-2 from different pools, our data consistently show that the vesicle-to-disk conversion after heat denaturation of apoA-2:DMPC disks is a slow reaction that takes hours to complete at 24°C
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, ascribed to it (Bryngelson et al, 1995; Dinner et al
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The sequence of fin-ray formation, characterized by initial development of fin elements, was caudal (7-8 mm BL), then pectoral (9-10 mm BL), anal (9-10 mm BL), dorsal (9-10 mm BL), and pelvic (10-11 mm BL)
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Hence, peak properties can be used to directly monitor the characteristics of individual molecular subpopulations under different solution conditions
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Effect of Nd: YAG laser energy on articular cartilage healing in the dog Lasers Surg Med 1989:9:595-601
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Exp Cell Res 1993:204:268-77
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. The goal in these theories is to explain why chain properties such as R^sub g^, R^sub e^, R^sub h^, II, B^sub 2^, scattering functions, and internal correlations obey nontrivial power laws in the EV limit (39)
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