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. The fluorescence from ANS and Bis-ANS fluorophores show strict environmental dependence and are essentially nonfluorescent in water but are highly fluorescent in hydrophobic environments
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- maxima GR by GdnHCl has been proposed (Rendon et al
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. Folding of the four domains and dimerization are impaired by the G/y446-Glu exchange in human glutathione reductase
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. 1628 cm^sup -1^ or the fluorescence intensity at 320 nm vs
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. Crystal structure of D-aminoacylase from Alcaligenes faecalis DA1
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. Conversely, zooming out or scaling up from the contact density map of a short protein like PSBD will yield the contact density maps of longer proteins such as ubiquitin or titin
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. The N-terminal domain showed basically the same structure as the EIN^sup ec^ (8,9), since the alignments for homology modeling showed only minor deletions in loops between α-helix I and α-helix 2, α-helix 2 and α-helix 3, and α-helix 3 and α-helix 4 (Fig
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. Similarly, collectin family members, including mannose-binding lectin, surfactant protein (SP)-A, SP-D, and the collectin-like Clq, opsonize apoptotic cells with their globular heads, and then form a complex through their collagenous tails with phagocyte calreticulin and LRP
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.Single-molecule methods represent an excellent tool to decipher the complexity of RNA folding processes, which generally proceed via intermediates and multiple pathways
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