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MATERIALS AND METHODS Materials Tris (hydroxymethyl) aminomethane buffer, [N-[2-hydroxyethyl]piperazine-N'-[2-ethanesulphonic acid]] (HEPES), and guanidinium hydrochloride were from Sigma Chemical (St
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Here, T is the desired temperature and Θ is the theta temperature, where polymers behave like ideal chains, and N is the chain length
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Internal correlations show scale invariance As noted in the introduction, the self-repelling nature of proteins in the EV limit imposes correlations on all length scales
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Since, EI^sup SC^ shows a sigmoidal thermal transition at neutral pH
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Unlike complement or Fc[gamma] receptor-mediated phagocytosis, efferocytosis and macropinocytosis are morphologically similar, and both are inhibited by amiloride
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It is widely accepted that pico- to nanosecond thermal fluctuations serve as a lubricant for functionally relevant motions occurring on a micro- to millisecond timescale (6)
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The delay between the 180° pulses (δ) in the CPMG was set to either 450 µs or 3
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87, showing that most residues are affected by the same contribution of nanosecondtimescale motion
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After amplification and discrimination, signals were directed to an ALV/5000E Multiple Tau Digital Correlator and time-intensity correlation functions recorded, typically for 600 s duration
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The concentration dependence of binding closely matches the activation profile (Runnels and Scarlata, 1998)
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The former may reflect the relative ease with which a smaller protein with fewer lipid-binding helices can dissociate from the disk as compared to a larger protein with multiple, possibly cooperative lipid binding sites
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The T^sub g^ of the Colorocs toner was reported to be 58°C [19]
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Risk spreading as an adaptive strategy in iteroparous life histories
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Thirty-four species of rockfishes: Maturity and seasonality of reproduction, Fish
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Thus, it becomes possible to identify P
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)19Fisheries Laboratory Late15 Jan '97of Kinki University(Kushimoto, Wakayama Pref
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We define this reference time as the average burst duration within the 0
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Antibodies to collagen type-II (CIICI-Developmental Studies Hybridoma Bank, University of Iowa) and collagen type-I (Sigma) were used at dilutions of 1 in 100 and 1 in 500, respectively
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