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Unfolding of GR by GdnHCl was further characterized by intrinsic fluorescence anisotropy measurements (Fig
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Mechanisms of Protein Folding
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Protein was still functional after the concentration step, as shown by binding experiments (28)
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Identification of nanosecond-timescale internal motionThe presence of nanosecond-timescale motion can be directly identified from a plot of the normalized ratio of R^sub 1^ values measured at two magnetic fields (RT^sup n^^sub 1^) versus NOE (22)
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For colloidal systems, the initial effects of attraction, particularly at high volume fractions, is a reduction in viscosity as the initial clustering of particles increases the free volume available for structural rearrangement (1)
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DCVJ bound to the early prefibrillar oligomers (300-500 kD) with an apparent dissociation constant of 1
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5 min): generation of the A-state monomer; 2), first aggregation phase (0
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These data were well approximated by mono-exponential functions, and the Arrhenius analysis yielded the activation energy E^sub a^ = 50 ± 5 kcal/mol, consistent with our estimate based on the scan rate effects on the melting data (Fang et al
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