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. After equilibration for 90 min in the presence of 3 M GdnHCl, major changes in fluorescence emission were observed
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. 31nm and that of gSBA is ~4
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. A new perspective on unfolded proteins
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. but decreased as the temperature was raised (data not shown)
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. 1 shows the change in ANS fluorescence induced by the four azurin species upon freezing of the sample at -13°C (200 µM ANS, 2
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. The whole-cell MAS spectrum being dominated by lipids is in apparent contrast with previous whole-cell experiments in another large single cell, an A
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. However, due to the close proximity of all involved components, a new CaM:SEF2-1mp interaction is rapidly reformed, which in turn forces the CaM dimer to close again
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. DNA compaction under such conditions can be considered as a model of DNA compaction in the protein-crowded environment inside living cells
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. ThT, on the other hand, requires a pH adjustment for its characteristic amyloid fluorescence signal (Fig
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. In this case the gutter-like structure survives a projection onto just two dimensions of folding space
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. The large number of these, of order N times greater than the number of native interactions, make them a likely candidate for information storage, as well as their natural propensity for kinetic control
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. , 1982; Elias and Burgos, 1988; Gonzalez, 1998)
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. Consider the short-chain fatty acids (SCFAs), one of which (butyric acid) is found almost exclusively in milk fat
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. 5), the entropy buildup curves show a substantial jump at the appending point when the trajectory of the free form is appended to that of the complex form (dashed line), indicating that the free molecule samples conformational space not visited in the complex simulation (case A; Fig
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. The parameters, such as polymer concentration and cooling rates for phase separation of polyethylene, were quite different from those used in our study to produce IPP and EVA toners
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. The two positive peaks located at 1618 and 1682 cm^sup -1^ confirmed the appearance of the aggregates observed in Fig
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