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The N-terminal domain showed basically the same structure as the EIN^sup ec^ (8,9), since the alignments for homology modeling showed only minor deletions in loops between α-helix I and α-helix 2, α-helix 2 and α-helix 3, and α-helix 3 and α-helix 4 (Fig
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When compared with the EIC^sup tt^
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For this purpose the same experimental setup (temperature controlling unit, quartz-cuvettes with 1 cm optical pathlength) was used as for fluorescence studies
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In vivo NMR Spectroscopy: Principles and Techniques
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Stress-strain curves of adsorbed protein layers at the air/water interface measured with surface shear rheology
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Several general predictions result: 1), intermediate subspaces of configurations will always be visited; 2), specific but nonnative interactions may be important in stabilizing these low-dimensional diffusive searches on the folding pathway, as well as native interactions; 3), sequential barriers will commonly be found, even in "two-state" proteins; 4), very early times will show characteristic departures from single-exponential kinetics; and 5), contributions of nonnative interactions to Φ-values and "Chevron plots" are calculable, and may be significant
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Phase separation occurred in the metastable region
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85, corresponding to donor-acceptor distances of ~64 [Angstrom] and 40 [Angstrom], respectively
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