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Meanwhile, small autopeaks could be identified at 1649 and 1658 cm^sup -1^, and a minor autopeak centered at 1682 cm^sup -1^ was below the cutoff of the plot and was not presented
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In a previous study, we probed the role of the two proximal GlcNAc residues in tethering the glycans attached to one subunit to the side chains of amino acid residues of an adjacent subunit at the noncanonical interface (18)
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4 using a contour plot of the two-dimensional distribution function ρ(R^sub g^/N^sup 0
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2-cm cell at a protein concentration of 0
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As this is not available one can at best calculate the minimum average number of binding sites per macromolecule, n, by assuming the maximum fluorescence intensity increment, ~110-fold, ever observed between free and protein-bound ANS (φ^sub Fprotein^/φ^sub Fice^ = 0
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They were calculated by dividing 5 ppm by the chemical shift span corresponding to the specified rf bandwith and the magnetic field
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In line with this interpretation, previous studies found an apparent mitochondrial asymmetry along the animal-vegetal axis of the Xenopus egg (35,36)
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Therefore, Pb, Ag, or Bi does not contribute to forming intermetallic compounds (IMCs) and only the Cu-Sn IMCs were found for both Sn-Pb and SnAg-Cu-Bi solders with Cu substrates, as shown in Fig
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Mass spectrometryThe BLG preparation was examined by liquid chromatography-mass spectroscopy using reverse phase chromatography with on-line electrospray-ionization mass spectrometry (MicroMass, Cary, NC) as described previously (14)
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Chemical cross-linkingA total of 100 µl of aliquots from a TTR misfolding reaction was withdrawn al different time points, and 100 mM phosphate buffer was added in addition to 10 µl of glutaraldehyde (25%, electron microscopy grade (Sigma, St
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5 B indicate that pressure does not promote the transition of PLCβ to an activated conformation
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de Beer, and D
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A few melanophores were scattered at the base of the pectoral fin bud
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