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On the other hand, tryptophan emission is quite sensitive to the polarity of the surrounding environment, and fluorescence red-shifts are indicative of increased exposure of tryptophan residues to the aqueous medium (Lakowicz, 1983) overnight xanax .
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However, upon unfolding transitions generally distinct shifts of the emission spectra are reported (see for example (4,9-14)) overnight xanax .
For PPA, which already exposes tryptophan residues to the protein surface in the folded state, a blue shift of the emission spectrum occurs upon thermal unfolding (see Fig overnight xanax .
Before comparing the behavior of individual proteins it is convenient to divide the group into two classes: one (β-gal, α-amylase, and ALD), which binds ANS essentially only in the frozen state, and the rest, which bind a fair amount of ANS also in liquid solution (4,5,28) overnight xanax .
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12 ml g^sup -1^) gives better agreement at higher protein concentrations (Fig overnight xanax .
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pH-independent binding and fluorescence of DCVJOne problem frequently encountered with ThT is the pH-dependent fluorescence in amyloid fibril formation measurements overnight xanax .
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