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Unfolding of GR by GdnHCl was further characterized by intrinsic fluorescence anisotropy measurements (Fig overdose symptom vicodin .
At temperatures above 70°C, all the bands except those from the β-turn (1668 and 1674 cm^sup -1^) possessed similar melting curves, which indicated that a major conformational change occurred in this temperature range overdose symptom vicodin .
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