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, glycans attached to subunit X forming hydrogen bonds with residues of subunit X), whereas the rest are intersubunit interactions
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Pathways of protein folding
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Contrary to interpretations of many such experiments, numerous molecular simulations (7,23,99) and recent single-molecule experiments (75) provide little evidence of long-range residual structure under harshly denaturing conditions
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uk) by using the Needleman-Wunsch global alignment algorithm (with the following parameters: matrix: EBLOSUM62, gap penally value: 10
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For PPA, which already exposes tryptophan residues to the protein surface in the folded state, a blue shift of the emission spectrum occurs upon thermal unfolding (see Fig
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It was sharply enhanced in the presence of the destabilizing salt KSCN or on lowering the temperature, whereas no fluorescence enhancement was found when azurin is stabilized by the addition of glycerol or by adding Cd^sup 2+^ to the metal binding site
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3 is achieved when only the average (τ^sup o^^sub m^)^sub app^ in the helices are considered
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The shortest incubation at 60°C (or 70°C) applied to insulin samples was only 30 s; yet as Fig
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So, when the high-dimensionality of the search space is retained, the energy landscape looks less like a funnel, and more like a series of high-dimensional gutters (Fig
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In contrast, the SL 4WJ shows a dramatic shift from very low E (0
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They may also remove heads, wings, and other parts of larger prey (along with diagnostic bones and larger feathers) prior to ingestion (Bosakowski et al
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Exp Cell Res 1997:237: 318-25
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