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Conversely, the contact density maps for the folded versions of different sequences reflect differences in native-state topologies mt mt tb.cgi trackback xenical .
Given access to contact density maps for the denatured state (EV limit) and contact maps for native states, one can make a qualitative judgment regarding the folding process by computing difference contact density maps between the native and denatured states mt mt tb.cgi trackback xenical .
Precisely how many and which nonnative local contacts are to be broken is determined by the native-state topology and hence the specific amino acid sequence mt mt tb.cgi trackback xenical .
thus indicating that the helical content of EIC^sup sc^ increased mt mt tb.cgi trackback xenical .
Its characteristic features are: i), specific high-affinity binding in the presence of fast exchange, ii), accommodation of a relatively nonmatching and flexible target via exchange between multiple binding alternatives, iii) dimerization of CaM upon target binding, and iv), large-scale (domain) motions on the nanosecond and microsecond timescale mt mt tb.cgi trackback xenical .
Aggregates are expected to have rotational correlation times considerably longer than the decay time of the used dyes (e mt mt tb.cgi trackback xenical .
It should be emphasized that this is not due to loss-of-fluorescence due to titration of ThT, which is the case for, e mt mt tb.cgi trackback xenical .
This suggests that the protein refolding detected by CD between 32 and 24°C upon relatively rapid cooling (Fig mt mt tb.cgi trackback xenical .
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