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Phase diagram analysis of IR and fluorescence data The phase diagram analysis, which is a sensitive tool to detect folding intermediates, was carried out as described previously (35) medication pain vicodin .
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Our results show that the secondary structure of EI^sup sc^ was not substantially altered, as suggested by FTIR experiments, but quenching experiments and proteolysis patterns suggest that there were small conformational changes upon binding of PEP; in addition, the thermal stability of EI^sup sc^ was slightly modified when any effector was added medication pain vicodin .
If, for example, a tryptophan residue becomes more accessible to the solvent, which quite often is the case for unfolded states, the emission intensity decreases medication pain vicodin .
For some proteins (BSA, YADH, ALD) the profile is distinctly Multiphasic, and in many cases (all but LDH and azurin) saturation is not reached up to 300 µM ANS medication pain vicodin .
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Different conformations of double-stranded nucleic acid in solution as revealed by circular dichroism medication pain vicodin .
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It also recorded the bet size, the total number of points sat on (total sat on), the number of points when the last card was drawn (total drawn on), and the number of play decisions that violated the Basic strategy (i medication pain vicodin .
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