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Folding and stability of El^sup sc^ in the absence of effectors Two unfolding mechanisms have been described in oligomeric proteins exposed to high temperatures or high concentrations of chemical denaturants at equilibrium (49-51): 1), dissociation followed by unfolding of the native or partially unfolded monomeric species; and 2), denaturation without the accumulation of the native dissociated species
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This value (311 ± 5 K, at 4 µM protein concentration, 315 ± 9 K, at 6 µM protein concentration) was similar, within the experimental uncertainty, to that obtained in the intact EI^sup ec^ (4), where two tryptophan residues are located at the C-terminal region of the protein
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Glover (United Kingdom), and F
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Although different distribution profiles of fibrillar features may still be explained in terms of kinetic effects (temperature may differently affect the kinetics at various stages of the assembly, effectively marginalizing certain sequential processes), the higher temperature may also have a more direct effect on amyloidogenesis, for instance by increasing the thermal energy of the interacting molecules and hence causing the alignment of protofilaments to become less accurate
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DiscussionDepartures from rational (Basic) behavior occur in blackjack but may simply reflect ignorance or fatigue
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