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The folding of ACYI has been characterized to be a three-state process involving an inactive dimeric intermediate with molten globule-like characteristics (18,19)
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The heat flow versus temperature profile of ACYI contained an asymmetric positive peak arising from an exothermic transition (Fig
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Beyond local length scales, chain properties follow well-defined power laws that are expected for generic polymers in the EV limit
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The ability to simulate denatured-state ensembles is important for a range of applications including protein design (25,26)
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Fingering IL-12 with apoptotic cells
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The fluorescence of tryptophan residues is very sensitive to the local environment
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In the experiments with samples with aggregated proteins, the monomeric form was mixed with a small contribution from considerably larger particles (giving essentially a baseline), making a detailed analysis difficult
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This probe becomes fluorescent when it reacts with cysteine side chains (see Molecular Probes Handbook, Eugene, OR)
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In the same way, if denaturant concentration affects the stability of the low-dimensional search spaces, or gutters, that are principally responsible for controlling the folding rate, then these experiments will also carry signatures of the search-structure
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Role of secondary structure elements in tertiary foldingThe systematic study of hairpin ribozyme constructs containing different combinations of helical junction and loop elements presented here enables us to define the role of these secondary structural elements during tertiary folding
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