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. The mutation will simply remain in the population
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. Irrespective of chain length and sequence, the contact densities follow a hierarchical pattern whereby near-neighbor residues have a higher probability of being spatially proximal
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. From the often drastic shortening of the phosphorescence lifetime of Tip residues buried within the rigid core of these macromolecules, it was deduced that their internal flexibility is greatly enhanced in ice, a response to freezing similar to that observed when the polypeptide loses much of its tertiary structure as in the formation of molten globule states
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. A homodimeric peptide corresponding to the complete SEF2-1 basic sequence, henceforth called SEF2-1mp, was chosen as a good model system for NMR studies of the interactions between bHLH proteins and Ca^sup 2+^/CaM
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. , K^sub d^(app) ~ 7 nM) previously determined by FRET (Runnels and Scarlata, 1998)
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. In the intermediate q regime, proteins in the EV limit show scale-invariant, sequence-independent behavior wherein properties such as chain size and internal distances follow well-defined power laws
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. The stability of these mutants is ~3 kcal/mole lower relatively to the WT (20-22), and the internal flexibility of the polypeptide is considerably larger, particularly with I7S and F110S (18)
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