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Secondary structure of proteins from circular dichroism spectra global pharmacy phentermine .
The enzymes were dissolved in 30 mM Tris-HCl, pH 7 global pharmacy phentermine .
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A similar difference in emission peak maxima between thermal and GndHCl-induced unfolding was also observed for other proteins (see for example (10,21)) global pharmacy phentermine .
Here, we compare the ANS binding capacity of the four azurin species to inquire how these modified features modulate the ice-induced perturbation of tertiary structure global pharmacy phentermine .
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The delay between the 180° pulses (δ) in the CPMG was set to either 450 µs or 3 global pharmacy phentermine .
The ratio of R^sup 2^ over R^sup 1^ (R^sup 2^ corrected for R^sub ex^; vide infra) at a magnetic field B gives the apparent correlation time, (τ^sup B^^sub m^)^sub app^ (Eq global pharmacy phentermine .
The Trp fluorescence of acid unfolded TTR peaks at 352 nm (data not shown) compared to 340 nm in the native protein, indicating unfolding global pharmacy phentermine .
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Structural analysis of the rate-limiting transition states in the folding of Im7 and Im9: similarities and differences in the folding of homologous proteins global pharmacy phentermine .
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