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. 5 M denaturant concentration also exibits a loss in the value of the mean residue ellipticity, suggesting that there is a loss in the secondary structure of the protein (Fig
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. The value of R^sub o^ obtained from fits to SAXS data for denatured proteins suggests that denatured proteins show rodlike behavior over very short length scales (11)
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. To illustrate these points, we compare the values of P^sub oa^(r) obtained in the EV limit to those for three different models
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. The intensities of the bands at different times were measured by densitometry
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. Am J Respir Crit Care Med 2005; 172:987-993(58) Mancini GBJ, Etminan M, Zhang B, et al
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. Outside of these conditions, proteins exhibit denatured and structurally unfolded states
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. The relationship observed between reduced protein flexibility and increased thermal stability is in line with other experiments on trehalose-coated myoglobin (56) and on lysozyme in glycerol (57,58)
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. On the other hand, globular proteins are generally nonspherical and there remain uncertainties in the prediction of interparticle forces
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. , 1999) to CPM-PLCβ gave a volume change similar to Gβγ-subunits (Table 1), which suggests that the contacts made by this peptide reflect the same ones made by the whole Gβγ-subunit (see Discussion)
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- Of the 75 tracks on the three-CD Essential Johnny Cash which The Legend effectively replaces, 32 do not appear on the new set
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. Measuring unfolding of proteins in the presence of denaturant using fluorescence correlation spectroscopy
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. In experiments aimed at detecting the burial of the aromatic residues upon binding to PEP and/or Mg^sup 2+^, the concentration of EI^sup sc^ was 4 µM at pH 7
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. Early measurements (42) showed BLG has a dipole moment of a sufficient magnitude to generate significant attractive dipole-dipole interactions which are predicted to be screened by increasing ionic strength at the concentrations relevant to this study (40,43)
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.This work was supported through the core strategic grant of the United Kingdom Biotechnology and Biological Sciences Research Council
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