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Since the frequency of the solvent-exposed secondary structures is smaller than the buried ones (44,45), the above sequential events could be interpreted as follows: the unfolding of the solvent-exposed β-sheet structures is the earliest event, followed by the formation of aggregates, and finally the unfolding of the other native structures er recall ultram .
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The models were constructed with the SWISS MODEL server (33) by using the structures of EIN^sup ec^ (PDB accession code IZYM) and EIC^sup tt^ (PDB accession code 2BG5) as templates er recall ultram .
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