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3), we used fluorescence quenching to further investigate the solvent accessibility of tryptophan residues upon unfolding effects side tramadol ultram .
Validity of the "two-state" hypothesis for conformational transitions for proteins effects side tramadol ultram .
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For all studies the following buffer was used: 30 mM Mops, 50 mM NaCl, 2 mM CaCl^sub 2^, pH 7 effects side tramadol ultram .
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