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Although gSBA unfolded via a two-state process without the formation of any unfolding intermedialcs, the denaturation profile of rSBA was poputated with an intermediate (Fig
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The aromatic chromophore 1-anilino-8-naphthalene sulfonate (ANS) is feebly fluorescent in water, but its spectrum is blue shifted and its intensity is dramatically increased in nonpolar solvents or when it binds to nonpolar sites of proteins (4,5; for a general review, see Slavik (6))
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By assuming equilibrium conditions and an ANS activity in ice equal to the concentration in solution multiplied by a freeze concentration factor of 120 (12), one derives ANS dissociation constants in the range of 6-8 mM for WT, 2-4 mM for C3A/C26A, 2
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After the buffer baseline subtraction, the data were normalized to protein concentration and expressed as molar residue duplicity, [Θ], No changes in [Θ] were detected with changes in the protein concentration in the range explored (5-600 µg/ml)
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Casing CaseinEighty percent of the protein in milk is casein
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We selected a dissolving temperature of 70°C
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There was no technical problem assaying 43 or 102 eggs from natural waters
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An epitope recognized by the monoclonal antibody MR-21 which is reactive with the cell surface of Chattonella marina type II
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6), 10 mM MgCl^sub 2^, and 5 mM dithiothreitol for 30 min at 377deg;C
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Such a procedure takes into account the shot noise-induced broadening of the peak (Deniz et al
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Food habits of the Great Horned Owl (Bubo virginianus) in the Cape Region of Eower California, Mexico
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t] x t + [[epsilon]
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Preferences in favorite game showed no difference between the two groups except that those under 21 years were more than twice as likely to prefer sports betting to those over 21
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