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Intrinsic fluorescence spectroscopy The samples for the fluorescence measurements were prepared by dissolved ACYI in 30 mM Tris-HCl (pH 7
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In high concentrations of denaturants, proteins behave like polymers in a good solvent and ensembles for denatured proteins can be modeled by ignoring all interactions except excluded volume (EV) effects
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5 mM PEP and 5 mM Mg^sup 2+^), and every digestion was repeated three times
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Surprisingly, little is known about these alterations, but it has become clear that the externalization of phosphatidylserine (PS) is an essential element
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The experiments were carried out on Bruker DRX-400, AMX2-500, and DRX-600 spectrometers equipped with triple-resonance (^sup 1^H/^sup 13^C/^sup 15^N or broadband) probes with XYZ-gradient capabilities
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Thirdly, the relaxation-compensated CPMG experiment at 600 MHz was repeated at a lower temperature (300 K instead of 308 K) to measure the change in ΔR^sub ex^, ΔΔR^sub ex^
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They can either be in fast exchange or in slow exchange to a lowly populated bound CaM state
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When both the amplitudes and the rates are taken into context, it is evident that ThT responds slowest of all probes in the study, which suggests that its strongest signal emanates from the most mature fibrils
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5 kbar (see Sassaroli et al
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LarvaeIchthyoplankton was sampled by using Bongo, Nackthai, and PairoVET nets
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These three situations, denoted cases A, B, and C, will be used in Results, below
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t] indicator equals 1 in April 1994 and thereafter (the period during which the Type 1 RP policy was in effect), and equals 0 otherwise; [RP2
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From the seven cards, a player makes his best five-card hand
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