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. INTRODUCTION Freezing of protein solutions may result in irreversible protein aggregation and severe loss of catalytic activity of enzymes (1), a phenomenon of basic concern and of growing commercial importance for the expanding polypeptide-based pharmaceutical industry
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. The time course of the protein unfolding, which was triggered by a rapid increase in the Gdm HCl concentration from 0 to 1-6 M, was monitored by CD at 222 nm (Fig
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. In this case the gutter-like structure survives a projection onto just two dimensions of folding space
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