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. In high concentrations of denaturants, proteins behave like polymers in a good solvent and ensembles for denatured proteins can be modeled by ignoring all interactions except excluded volume (EV) effects
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. Hence, this quasi-docked species must also form in the native 4WJ ribozyme, possibly as a precursor to the specifically docked native state (Fig
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. In contrast, the specifically-docked conformation (exemplified by the WT 4WJ) is stabilized by an extensive hydrogen bond network (14 hydrogen bonds in all (Rupert and Ferre-D'Amare, 2001)), prolonging the lifetime of this state
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