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1 B) at wavelengths ~208 nm
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In the case of multitryptophan proteins, tyrosine fluorescence emission is generally not visible, mainly due to much larger absorption coefficients and quantum yields of the tryptophans, and at least for folded states because of distinct Förster energy transfer from tyrosines to tryptophans (3,14)
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For most of the peaks in the histograms, the peak position (E^sup peak^) from these fits is a good approximation for the mean E for the peak, due to their fairly symmetric shapes
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, under conditions used for generating the FRET histograms in this article)
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