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4, containing 1 mM Ca^sup 2+^/Mn^sup 2+^ depression zyban .
The column was then washed extensively with PBS till the washings had A^sub 280^Isothermal guanidinium-hydrochloride-induced denaturation Equilibrium unfolding as a function of guanidinium hydrochloride (GdnCl) concentration was monitored by fluorescence spectroscopy depression zyban .
coelicolor, EI^sup sc^, indicate that its thermal denaturation behavior is different from that of other EI proteins, since only one transition is observed (23) depression zyban .
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To summarize the above described observations one can state, that GndHCl-induced unfolding reveals for all α-amylases rather uniform emission spectra with large red shifts, whereas thermal unfolded states appear with more divers and in general with much smaller λ^sub max^-values as compared to the former depression zyban .
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The histogram shows two well-resolved (non-zero) peaks, centered at E ~ 0 depression zyban .
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