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For large x, entropy opposes stretching of the chain beyond its average value of R^sub e^ compare prices on ultram .
Thermal denaturation measurements Experiments were performed with a response time of 8 s and at constant heating rates of 15, 30, and 60 K/h to test for microreversibility (6), in the presence of 1 mM β-mercaptoethanol compare prices on ultram .
Thus, weakening the azurin fold by the mutations leads to the creation of additional binding sites characterized by a broad distribution of binding affinities, a large fraction of which are of stronger affinity than in the WT protein compare prices on ultram .
Furthermore, several residues in CaM also undergo conformational exchange on a timescale of ~50 µs compare prices on ultram .
H-1, C-13 and N-15 chemical-shift referencing in biomolecular NMR compare prices on ultram .
There are four known families of PLCβ enzymes and all are activated by Gα^sub q^-subunits (for review, see Rebecchi and Pentyala, 2000; Rhee, 2001) compare prices on ultram .
, 1981), lipid binding increases the helix content in apoA-2 to ~64% compare prices on ultram .
The rationale of this work was to inquire into the hierarchical assembly of the insulin amyloid and link the morphological transitions to conformational events observed by other biophysical and spcctroscopic methods compare prices on ultram .
Alternatively, our picture suggests ways of increasing folding times greatly by selective mutations that retain topology and stability of the native state, but destabilize one or more of the on-pathway diffusional subspaces so that intermediate searches are required in d > 3 compare prices on ultram .
Careful double mutant studies would be required to identify such nonnative contributions unequivocally compare prices on ultram .
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The interaction was not significant, F (1,140) = compare prices on ultram .
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