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The presence of Mg^sup 2+^ slightly increased the T^sub m^ of EI^sup sc^ alone, probably due to formation of an octahedral-coordination-geometry complex to the native state of EI^sup sc^, as occurs in other homologous proteins (56,57) cialis story success .
At the moment, however, we do not know whether these small changes in the structure are also responsible for the observed considerable specificity of the phosphotransfer reaction between EI and HPr among the different species and the differences in their cnthalpic variations in their binding reactions (45 cialis story success .
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They are clustered in the CaM:SEF2-1mp binding interface, the CaM:CaM dimer interface, and in the flexible helix connecting the CaM N- and C-terminal domains, and have similar exchange times (~50 µs), suggesting a cooperative mechanism probably caused by protein:protein interactions cialis story success .
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