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Apparently, highly denatured states are to be viewed as embryos of native states since it is expected that native-like local and/or nonlocal signals are sampled with statistically significant probability in the denatured-state ensemble (10,130,131)
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Oxford University Press, Oxford, UK
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Native proteins are surface-molten solids: application of the Lindemann criterion for the solid versus liquid state
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Persistence of native-like topology in a denatured protein in 8M urea
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Mass normalized fluorescence profiles are shown in Fig
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However, due to the close proximity of all involved components, a new CaM:SEF2-1mp interaction is rapidly reformed, which in turn forces the CaM dimer to close again
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Our main goal with this study was to employ a variety of fluorescence probes (ANS, Bis-ANS, DCVJ, and ThT) and techniques (steady state and time resolved) to recognize and characterize different states of a protein in the process of misfolding and self-assembly
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5 A, we present results showing that the affinity between CPM-PLCβ and Gβγ at atmospheric pressure on lipid membranes is strong and matches the K^sub d^(app) (i
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, 2000; Rye and Barter, 2004; Jayaraman et al
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Control samples (freshly cleaved mica, mica, and aqueous HCl-solution) were also investigated with AFM to exclude possible artifacts
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, Liquid-liquid Phase Separation and Crystallization in Binary Polymer Systems, Polymer 28 (2), 193-200 (1987)
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DNA levels remained fairly static in pellets cultured in DMEM/ 10%FCS, but increased throughout the culture period for pellets cultured in DMEM/ITS+/TGF-[beta]1
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