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J Exp Med 2000; 191:515-528(18) Okazawa H, Motegi S, Ohyama N, et al
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In this work we report a case study on five homologous α-amylases aiming to elucidate the impact of protein aggregation and conformational scrambling of the unfolded states on fluorescence emission
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The fluorescence emission of the folded state is already rather blue shifted (λ^sub max^ = 327 nm), but also rather large λ^sub max^-values (341-344 nm) for the unfolded states contribute significantly to the large red shift
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Comparison of solute-induced protein stabilization in aqueous solution and in the frozen and dried states
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Nonequilibrium Phase and Glass Transition Behavior of [beta]-Lactoglobulin, TheParker, RogerABSTRACTConcentrated solutions of bovine β-lactoglobulin were studied using osmotic stress and Theological techniques
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Data analysisConversion of the apparent K(d) obtained on membrane surfaces to the K(d) that would be obtained if the proteins were freely diffusing has been previously treated by assuming that the proteins are confined to interact within a reduced surface volume (ν) that is equal to the surface area of the membrane multiplied by the distance from the membrane surface into the aqueous phase where the proteins interact, which is taken to be 50 nm
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