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Since it has been widely recognized that changes in the conformation of DNA into higher density forms is the result of DNA negative charge neutralization (Bloomfield, 1991), the activity of cationic molecules in neutralizing DNA negative charge (through binding with DNA phosphates) has been associated with the potential to induce DNA phase separation processes, such as compaction, condensation, aggregation, and precipitation, also without clear distinctions between these different processes
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Far-UV CD spectra show that TTR under these conditions has close to a random coil conformation with a featureless spectrum and a minimum at 200 nm (Fig
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We note here that although we have interpreted peak shifts and broadening in terms of distance changes and ribozyme subpopulations, respectively, peak shifts could also potentially be caused by solvent effects on dye photophysics, and corresponding changes in the correction factor [gamma] (see Materials and Methods), as well as peak broadening by effects of [kappa]^sup 2^, the orientation factor (Deniz et al
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