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A further increase in quenching efficiency was observed at 7 M GdnHCl (squares, K^sub SV^ = 40 M^sup -1^), indicating that even at 3 M GdnHCl the tryptophan residues of GR were still significantly protected from iodide quenching relative to the fully unfolded protein
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The amide I' region of native ACYI was dominated by bands from the α-helix (1651 cm^sup -1^), α-helix and/or 3 m helix (1660 cm^sup -1^), β-sheet (1629, 1637, and 1682 cm^sup -1^), β-turn (1668 and 1674 cm^sup -1^), and random coil (1644 cm^sup -1^)
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