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Since the folding process is complex in multidomain proteins, where each domain may be able to refold independently and interdomain interactions may affect the overall folding process, EI can be used, in addition, as a model protein to improve our knowledge of protein folding and assembly of multidomain-oligomeric proteins
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Quenching experiments In experiments aimed to detect protein self-association, the concentration of EI^sup sc^ was varied at pH 7 from 0
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Respir Med 2005; 99:1175-1182(65) Berry MA, Hargadon B, Shelley M, et al
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In either case the conclusion is that in the frozen state the polypeptide structure of the putative binding sites has changed, becoming unable to host ANS or to enhance its fluorescence yield, as when the probe surrounding changes from rigid to mobile (26)
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